Veterinary Medicine and Public Health Journal

Volume 1 - Issue 3 (2) | PP: 81 - 84 Language : English
DOI : https://doi.org/10.31559/vmph2020.1.3.2
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Investigating the effects of theophylline on motility, hyperactivity, and acrosome reaction of spermatozoa in rams

K.H. El-Shahat ,
Taysser M. I ,
M.R. Badr ,
K. Zaki
Received Date Revised Date Accepted Date Publication Date
24/4/2020 7/6/2020 15/6/2020 29/7/2020
Abstract
The present study was conducted to evaluate the effect of in vitro treatment of ejaculated ram spermatozoa with different levels of theophylline at various incubation times on motility, hyperactivity (HA), and acrosome reaction (AR). Semen was collected from Barki rams (n=40 ejaculates) using an artificial vagina twice a week. Collected semen were pooled and subjected to swim up technique in modified sperm Tyrode's albumin lactate pyruvate (S-TALP) medium supplemented with different levels of theophylline (0.01, 0.02, 0.03, 0.04, and 0.05 mg/ml) at different incubation times (0, 1,2,3 and 4 hours). The following parameters were examined: motility, hyperactivity (HA), and acrosome reaction (AR %). The results revealed that the highest motility was noticed in spermatozoa treated with 0.05 mg/ml theophylline (82.53&plusmn;1.34%) immediately after dilution. While the addition of 0.01 mg/ml theophylline resulted in a significant (P<0.05) increase in HA% than those obtained in control (untreated) after 3 hours of incubation (5.04&plusmn;0.26 vs.0.8&plusmn;0.2). On other hand, a significant (P<0.05) increase in total AR% was concomitant to the increase in the concentration of theophylline with a maximum achieved at 0.05 mg/ml at 3- and 4-hours post-incubation (49.2&plusmn;1.8; 51.2&plusmn;2.5, respectively). In conclusion, under our experimental conditions, treatment of ram spermatozoa with 0.05 mg/ml of theophylline for 3-4 hours was considered the best concentration of theophylline to be used for in vitro induction of acrosome reaction.


How To Cite This Article
, K. E.I , T. M. , M. B. & , K. Z. (2020). Investigating the effects of theophylline on motility, hyperactivity, and acrosome reaction of spermatozoa in rams . Veterinary Medicine and Public Health Journal, 1 (3), 81-84, 10.31559/vmph2020.1.3.2

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