In this study the aim is to investigate aflatoxin M1 (AFM1) contamination levels in fluid milks offered for sale in Khartoum State, Sudan. Different milk samples were analyzed by aflasensor kits, using screening method; about 25 samples from raw and 62 samples of processed milk from 3 factories; 40 UHT, 12 flavored UHT and 10 pasteurized milk. The results showed that 85.06% of milk samples were contamination with aflatoxin M1 at higher levels than those set by European Commission and Codex Alimentarius. High prevalence (88.7%) of aflatoxin M1 was detected in flavored, pasteurized and UHT revealed 100%, 100% and 82%, respectively and the raw milk showed 92%. In conclusion this study suggested that the presence of high contamination of AFM1 in the samples of fluid milk products could constituted possible risk hazards for human health. Therefore, imposing regulatory limits is needed for Sudan.